In the first scientific report of its kind, researchers in Arkansas showed that chickens bred for water conservation continued to put on weight despite heat stress that would normally slow growth.

Research by the Arkansas Agricultural Experiment Station indicates the specially bred line of chickens developed by Sara Orlowski could save growers thousands of gallons of water and thousands of pounds of food each month without sacrificing poultry health. Orlowski is an associate professor of poultry science with the U of A System Division of Agriculture.

As global population increases and usable water diminishes due to climate change patterns, scientists with the Division of Agriculture are looking for ways to raise the world's most popular meat protein using fewer resources.

The study, which was part of a five-year project funded by a $9.95 million grant from the U.S. Department of Agriculture's National Institute of Food and Agriculture, showed a broiler chicken's physiology could be significantly improved to convert food and water to body weight even with three weeks of heat stress. 

Results from the study were published in Physiological Reports, the American Physiological Society's scientific journal, as an article titled "Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines." The grant was awarded through NIFA's Agriculture and Food Research Initiative. 

Sami Dridi, professor of poultry science specializing in avian endocrinology and molecular genetics, was responsible for conducting the experiment and the driving force in writing the paper. 

Walter Bottje, professor of poultry science for the experiment station, is the project director for the USDA Sustainable Agriculture Systems multi-institutional grant led by the Center of Excellence for Poultry Science.

Now in its fifth generation of selection, the high water-efficient line has been measured to consume significantly less water than standard broiler lines in use today. From the time they were hatched to one month old, the high water-efficient line drank 1.3 pounds less water and about 5.7 ounces less feed, which calculates to a 32-point improvement in water conversion and six-point improvement in feed conversion when compared to a random-bred control line.

While it may not seem like a huge difference, modern chicken houses hold on average 20,000 to as many as 50,000 birds. Although chickens consume more as they grow, the difference for that month of growing equates to 7,800 fewer gallons of water and 17,800 pounds less feed to grow 50,000 water-efficient chickens.

In some conditions, the high water-efficient chicken had food conversion ratios that were just as good or better, and their water conversion ratio was about 55% to 65% better, according to Dridi.

Bottje said these recent results from the ongoing research are promising, but the group aims to investigate other physiological characteristics of the high water-efficient line, such as meat quality and gut integrity.

Thirst control

The hypothalamus is the part of the brain that controls thirst. One of the study's findings was that the hypothalamus of water-efficient chickens differed from the other chickens when exposed to heat stress. The investigation revealed potential molecular signatures for water efficiency and heat tolerance in chickens.

The researchers devised a study that induced heat stress for groups of chickens by increasing the ambient temperature to mimic a summer season in Arkansas. The heat-stress cycle began when the birds were 29 days old. The environment was also kept between 30% and 40% relative humidity.

Dridi ran a parallel study comparing data on the divergent lines of chickens.

What they found was surprising.

"What the most interesting thing from that study, when it comes to live performance, is that the heat-stressed birds from the high water-efficient line consumed less water than the non-heat stressed birds from the low water-efficient line," Orlowski said. 

Water is critical to raising chickens. They can go several days without food, but only a few hours without water at high temperatures, Dridi said.

Dridi said high humidity, which would be over 70% for chickens, also induces similar heat stress because the chickens cool themselves by breathing. Dridi's studies on poultry house sprinkler systems kept the humidity lower than the industry standard method and used significantly less water than evaporative cooling cells.

"With water sprinkling systems that can save up to 66% water usage in a poultry house, the water conservation of poultry could be improved by a magnitude of three- to four-fold by having chickens that consume less water and still retain growth," Dridi said.

Project development

Dridi said the idea for water-efficient chickens came from looking at the differences in chicken lines bred as far back as the 1950s. Dridi and other researchers wanted to see how much genetic differences there were between jungle fowl and modern breeds.

Before they could breed water-efficient chickens, though, they had to reliably measure the amount of water chickens drank.

Orlowski was a Ph.D. student when her graduate research team developed a novel low-flow water monitoring system in collaboration with Siloam Springs-based companies Alternative Design and Cobb-Vantress Inc., a primary broiler breeder company. The tool was essential to accurately measure water intake for individual birds in real time.

"When we first started this project in 2018, we evaluated one of our broiler lines, a non-selected control population, and we characterized them for water intake," Orlowski said. "And within that population there was a variability for water intake. From there, we were able to take our most water-efficient families and our least water-efficient families, establish our research populations and continue to select from there."

A base population of chickens that were not selected for high or low water-efficiency was kept as a control group to compare changes in each generation, Orlowski noted.

Bottje and Dridi said the work done by Orlowski in selecting the divergent lines of chickens was the most important factor of this experiment. Orlowski said water efficiency in the high water-efficient line is continuing to improve with each succeeding generation. She ranks the water efficiency trait as "moderately heritable."

"There's no reason that it will not work for all poultry operations, including turkeys, quail and ducks," Dridi said.

The researchers

The lead author on the research article was Loujain Aloui of the Higher School of Agriculture of Mograne at the University of Carthage in Zaghouan, Tunisia, while on an internship with the Center of Excellence for Poultry Science and the Division of Agriculture. 

Co-authors included Elizabeth S. Green, Travis Tabler, Kentu Lassiter, Bottje, Dridi and Orlowski with the Center of Excellence for Poultry Science at the Division of Agriculture and Kevin Thompson with the Center for Agricultural Data Analytics with the Division of Agriculture.

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To learn more about Division of Agriculture research, visit the Arkansas Agricultural Experiment Station website: aaes.uada.edu. Follow on Twitter at @ArkAgResearch. To learn more about the Division of Agriculture, visit uada.edu. Follow us on Twitter at @AgInArk. To learn about extension programs in Arkansas, contact your local Cooperative Extension Service agent or visit www.uaex.uada.edu. 

About the Division of Agriculture: The University of Arkansas System Division of Agriculture's mission is to strengthen agriculture, communities, and families by connecting trusted research to the adoption of best practices. Through the Agricultural Experiment Station and the Cooperative Extension Service, the Division of Agriculture conducts research and extension work within the nation's historic land grant education system. The Division of Agriculture is one of 20 entities within the University of Arkansas System. It has offices in all 75 counties in Arkansas and faculty on five system campuses. The University of Arkansas System Division of Agriculture offers all its Extension and Research programs and services without regard to race, color, sex, gender identity, sexual orientation, national origin, religion, age, disability, marital or veteran status, genetic information, or any other legally protected status, and is an Affirmative Action/Equal Opportunity Employer.