Seminar on AIMRC Imaging and Spectroscopy Core and Bioenergetics Core Resources

Arkansas Integrative Metabolic Research Center

Narasimhan Rajaram and Suresh Thallapuranam

The Arkansas Integrative Metabolic Research Center will host a Zoom seminar at 11 a.m. on Wednesday, Oct. 26, featuring Narasimhan Rajaram and Suresh Thallapuranam, directors of the AIMRC Imaging & Spectroscopy Core and the Bioenergetics Core, respectively.

The Arkansas Integrative Metabolic Research Center is an NIH-funded Center of Biomedical Research Excellence (COBRE) that was established in April 2021 at the U of A to study metabolism in cells and tissue. As part of the AIMRC, three research cores have been established as fee-for-service resources: an imaging and spectroscopy core, a bioenergetics core and a data science core.

This seminar will present the technologies and capabilities available within the imaging and spectroscopy and bioenergetics cores for utilization by universities and industry. The imaging and spectroscopy core currently houses state-of-the-art microscopes that allow high-resolution visualization of cell and tissue structure, function and biomolecular composition. Two-photon microscopy enables quantification of cellular metabolism through endogenous fluorescence intensity and lifetime of the metabolic coenzymes, NADH and FAD.

The bioenergetics core lodges cutting edge technologies to measure various aspects of cellular respiration and real-time metabolic analysis. The Oroboros O2k-FluoRespirometer provides a distinctive high-resolution approach to monitor cellular and mitochondrial respiratory function. In addition, the O2k-FluoRespirometer has the extraordinary capability to measure H2O2 flux, mt-membrane potential and ADP-ATP phosphorylation. Further, the Seahorse XFe8 /24 Analyzers, housed in the bioenergetics core, facilitate the measurement of key cellular functions such as mitochondrial respiration and glycolysis by measuring the oxygen consumption rate and the extracellular acidification rate of live cells.

This seminar will present an overview of each technology currently available in the cores, potential applications and details on how to access and/or get trained to use the equipment.

If you have any questions about this event, please contact Kimberley Fuller (fullerk@uark.edu).

This event is supported by the NIGMS of the National Institutes of Health under Award Number P20GM139768. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.