Paolo Actis of the University of Leeds will give a virtual seminar titled "Single-cell manipulation with single molecule resolution" on Zoom from 3:30-4:30 p.m. Friday, Jan. 29. The talk is free and open to the public.

Actis is a University Academic Fellow at the University of Leeds in the School of Electronic and Electrical Engineering. He is developing nanoprobes for single-cell analysis and manipulation with single molecule resolution.

After graduating with a Ph.D. in electrochemistry from the Grenoble Institute of Technology (FR), he spent four years in California at NASA Ames and University of California Santa Cruz before crossing the pond again to Imperial College London (UK). He then spent two years at Bio Nano Consulting as a consultant and project manager.

The delivery of molecules into cells is indispensable for the study of cell function in health and disease. A number of methods have been developed to introduce macromolecules into cells. Transfection of cells with nucleic acids is routine, but delivery of proteins, particularly when in biomolecular complexes, is far more challenging.

Actis will discuss the development of a single-molecule injection platform based on nanopipettes integrated into a scanning probe microscopy setup to enable the manipulation of living cells with single molecule resolution.

He will also present unpublished data to show how the crowded intracellular environment enhances the detection of single molecules.

To attend the seminar, please visit the Zoom link.
Meeting ID: 816 8890 5611
Passcode: Anyl@2021