NIH Grant Will Advance Study of Chronic Wound Biomarkers

In this three-dimensional reconstruction of skin, acquired through multi-photon microscopy, the outer layer, or epidermis, is identifiable through the natural auto-fluorescence of NADH, FAD and keratin, as seen in blue and green. The dermal layer is detectable in red.
FAYETTEVILLE, Ark. — The National Institutes of Health awarded a University of Arkansas biomedical engineer a new $744,992 grant to improve imaging and early detection of chronic wounds and guide treatments.
Kyle Quinn, assistant professor of biomedical engineering, uses multi-photon microscopy to create 3-D, micro-scale images of chronic wounds to measure different wound properties. The images reveal the metabolism and structure of skin layers and wound regions so that physicians can diagnose chronic wounds and determine appropriate treatment.
Chronic, non-healing wounds are caused by poor circulation, neuropathy, immobility and other factors. They affect millions of Americans and require advanced care with annual costs in billions of dollars. Infection is often a problem, and mortality rates for people with chronic wounds can exceed that of many cancers. Clinicians struggle because there are not optimal methods to diagnose a wound or evaluate appropriate therapeutic interventions.
“It’s currently a wait-and-see approach,” Quinn said. “After an initial diagnosis or checkup, the patient will come back weeks later, and the clinician will evaluate whether the wound has closed or begun closing. If it hasn’t, then the clinician will have to try some other treatment.”
With multi-photon microscopy, Quinn can exploit the intrinsic fluorescence of NADH and FAD, two cellular metabolic cofactors found in chronic wounds. These cofactors are necessary for most metabolic pathways. Multi-photon microscopy allows Quinn to build images of wound sections so he and others can detect and assess metabolic changes.
Data from a pilot phase of the research, funded by an earlier NIH award and accepted for publication in the Journal of Investigative Dermatology, indicated the ability to see differences in the metabolism of diabetic and non-diabetic wounds in frozen tissue samples. With the new grant, Quinn will non-invasively monitor individual wounds in live animals throughout the healing process. By the end of the three-year grant, he hopes to develop a suite of quantitative biomarkers to predict wound chronicity and to guide therapy. The long-term goal is to test the method in a clinical setting.
About the University of Arkansas: The University of Arkansas provides an internationally competitive education for undergraduate and graduate students in more than 200 academic programs. The university contributes new knowledge, economic development, basic and applied research, and creative activity while also providing service to academic and professional disciplines. The Carnegie Foundation classifies the University of Arkansas among only 2 percent of universities in America that have the highest level of research activity. U.S. News & World Report ranks the University of Arkansas among its top American public research universities. Founded in 1871, the University of Arkansas comprises 10 colleges and schools and maintains a low student-to-faculty ratio that promotes personal attention and close mentoring.
Contacts
Kyle Quinn, assistant professor, Department of Biomedical Engineering
College of Engineering
479-575-5364,
kpquinn@uark.edu
Matt McGowan, science and research communications officer
University Relations
479-575-4246,
dmcgowa@uark.edu
Headlines
U of A United Way Campaign Set to Begin Soon
This year’s U of A United Way Campaign begins Oct. 2 and runs through Nov. 3. Renewals and new donations will be accepted online throughout the campaign.
Adult and Lifelong Learning Student Recognized as Top 10 Civics Teacher of the Year Award Finalist
After 21 years of teaching in the public school system, Jessica Culver was recently recognized as a top 10 finalist for the Bill of Rights Institute's nationwide Civics Teacher of the Year Award.
Medical Humanities Sponsors Talk on History of Mumps
The Medical Humanities Program and Department of History will host a talk from Professor Matthew P. Romaniello of Weber State University on Oct. 6 from 11:50 a.m.-12:50 p.m. in Gearhart Hall 130.
National Leaders to Discuss U.S. Child Labor Laws
The Arkansas Law Review's annual symposium will feature 13 scholars, lawyers and child labor experts during sessions on Oct. 13 in the law school's E.J. Ball Courtroom. Register to reserve a seat.
Celebrate National Hispanic Heritage Month With the University Libraries
In celebration of National Hispanic Heritage Month, Mullins Library staff have compiled a list of ebooks and streaming videos available to all students, staff and faculty. Stop by Mullins Library to see the display, too.